The impact of microstructure and extracellular matrix suspension on the proliferation of bone marrow-derived mesenchymal stem cells for osteochondral defect repair.

ABSTRACT: Osteochondral defects are a challenge in orthopaedic surgery due to the complexity and function of cartilage. Within this scenario, this study aimed to develop/characterize bioactive porous supports based on oxidized polyvinyl alcohol (OxPVA), with/without human cartilage-derived decellularized ECM (dECM), as platforms for HM1-SV40 cell adhesion and proliferation. OxPVA scaffolds were fabricated using a particle-leaching technique (gelatin concentrations: 10%, 15% and 25% w/w); Scanning Electron Microscopy (SEM) was used to examine the ultrastructure, and a morphometric study assessed pores number, size and porosity percentage. Fluorescence Recovery after Photobleaching (FRAP) was used to evaluate the interconnectivity of the scaffold pores. To enhance the bioactivity of OxPVA, dECM (25% w

SUBMITTER: Stocco E 

PROVIDER: S-EPMC12718104 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature