Digestibility of Natural and Recombinant Allergenic Peanut Proteins in Artificial Gastrointestinal Fluids.

ABSTRACT: Safety assessments are necessary for genetically modified foods in many countries, including Japan. Stabilities during pepsin, trypsin, or pancreatin digestion are a key criterion for assessing the allergenic potential of newly expressed proteins (NEPs). In digestibility tests, NEPs produced by heterologous expression systems were frequently used. Polyhistidine tags (His-tags) are primarily/often used to purify recombinant proteins. Studies of His-tags' influences remain limited on the susceptibility of a protein to pepsin/trypsin digestion, although His-tags can affect protein folding and stability. In this study, we compared the digestibility of the natural peanut allergenic proteins Ara h 1 and Ara h 2 to the recombinant Ara h 1 protein with N-terminal His-tag and recombinant Ara h 2 pr

SUBMITTER: Terashima M 

PROVIDER: S-EPMC12718106 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature