A non-affinity-dependent high-resolution mass spectrometry method for detecting and typing monoclonal free light immunoglobulin chains.

ABSTRACT:

Background

Detecting and monitoring monoclonal free light immunoglobulin chains in serum is important for managing patients with B-cell neoplasms. Established methods have relied on immunochemistry, with monoclonality determined through an abnormal ratio of free kappa and lambda chains and an increased concentration of the involved chain. This indirect approach has limitations. Mass spectrometric methods that directly demonstrate the monoclonal fraction have been described; however, all reported approaches so far are affinity-dependent. The aim of this study was to develop a non-affinity-dependent high-resolution mass spectrometry (HRMS) method.

Methods

Samples were prepared using ultrafiltration, then separated by reversed-phase liquid chromatography and analyzed by HRMS. T

SUBMITTER: Bengtson P 

PROVIDER: S-EPMC12718204 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature