Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats.

ABSTRACT:

Objective

Knee osteoarthritis (KOA) is the most prevalent subtype of arthritis, characterized by progressive degeneration of articular cartilage. The present study aimed to investigate the reparative potential of exosomes derived from hypoxia-preconditioned bone marrow mesenchymal stem cells (Hypo-BMSCs-Exos) in treating cartilage damage associated with KOA.

Methods

An in vitro KOA chondrocyte model was established through induction with interleukin-1β (IL-1β). Subsequently, the modeled chondrocytes were co-cultured with Hypo-BMSCs-Exos. Flow cytometry, Western blotting, immunofluorescence staining, and senescence-associated β-galactosidase (SA-β-gal) staining were used to evaluate the effects of Hypo-BMSCs-Exos on chondrocyte proliferation, apoptosis, extracellular matrix (

SUBMITTER: Zhang B 

PROVIDER: S-EPMC12718206 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature