A Novel Salicylaldehyde Dehydrogenase from Alpine Soil Metagenome Reveals a Unique Catalytic Mechanism.

ABSTRACT: Metagenomic approaches have revolutionised the discovery of novel enzymes with ecological and biotechnological significance from different environments. Here, we report the comprehensive characterisation of a novel salicylaldehyde dehydrogenase (SALD_AP) obtained from an alpine soil metagenome. Phylogenetic analysis revealed that SALD_AP is the first experimentally characterised Alphaproteobacterial SALD, forming a distinct evolutionary clade among known bacterial enzymes. The recombinant enzyme exhibited strict specificity for NAD⁺ and exceptional catalytic efficiency toward aromatic aldehydes, with benzaldehyde as the preferred substrate. Kinetic analyses showed catalytic efficiencies exceeding 10⁶ M⁻¹ s⁻¹ for aromatics, whereas aliphatics were oxidised with much lowe

SUBMITTER: Dandare SU 

PROVIDER: S-EPMC12718228 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature