Toluene-2-monooxygenase expression and trichloroethene oxidizing activity of Burkholderia vietnamiensis G4 grown on benzyl alcohol and benzyl esters.

ABSTRACT: Benzyl alcohol (BA) and two BA esters were examined for their ability to support growth of Burkholderia vietnamiensis G4 and expression of toluene-2-monooxygenase (T2MO) activity. Resting cell assays and an activity-based labeling (ABL) approach were used to quantify T2MO activity and levels of T2MO hydroxylase subunit in cells grown either on substrates already known to support T2MO expression (toluene, phenol); novel aromatic substrates (BA, benzyl acetate (BAc); benzyl butyrate (BBu)); and non-aromatics (lactate, acetate, and butyrate). Specific rates of TCE oxidation and levels of activity-based fluorescent labeling of T2MO hydroxylase α-subunits in cells grown on BA or BA esters were comparable to or greater than those of cells grown on toluene or phenol. In contrast, levels of activi

SUBMITTER: Saito AM 

PROVIDER: S-EPMC12718231 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature