Identification of strong constitutive promoters in Burkholderia stagnalis TBRC 18363 for activating natural product production in Gram-negative bacteria.

ABSTRACT: Gram-negative bacteria are emerging as an important source of natural products with pharmaceutical potential. However, the limited availability of genetic tools for drug discovery and sustainable production of secondary metabolites remains a challenge. Burkholderia spp. serve as a promising source for such tools, as these bacteria produce diverse natural products and are amenable to genetic modification. We sequenced the genome of Burkholderia stagnalis TBRC 18363 and performed transcriptomic analysis to identify genes highly expressed in early to late exponential cultures. We hypothesized that the sequences upstream of the most highly expressed genes contain strong and constitutive promoters active in heterologous Gram-negative hosts. Twenty-six B. stagnalis TBRC 18363 promoters were eval

SUBMITTER: Wongsombat C 

PROVIDER: S-EPMC12718240 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature