Quantitative assessment of porin-mediated solute transport in biomimetic membranes.

ABSTRACT: Porins govern nutrient uptake and antibiotic influx in Gram-negative bacteria, making their characterization critical for understanding permeability, resistance mechanisms, and structure-function relationships. From a biotechnological point of view, they are effective tools for modulating the transport of substances across the outer bacterial membrane or for building catalytically active nanoreactors and biosensors. Quantitative data on mass transport through membranes is of great interest, but not trivial to obtain, as in vivo analyses are confounded by cellular complexity and variability. Here, we present a synthetic bottom-up approach, based on polymersomes containing reconstituted purified porins, enabling direct, quantitative measurement of substrate translocation, while minimizing in

SUBMITTER: Scherer M 

PROVIDER: S-EPMC12718255 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature