Double-antigen sandwich ELISA based on S1 for cross-species detection of Porcine deltacoronavirus antibodies.

ABSTRACT: Porcine deltacoronavirus (PDCoV) is an emerging enteric coronavirus causing high mortality in neonatal piglets and posing a significant threat to the swine industry. Evidence indicates that PDCoV has cross-species transmission potential and may pose a zoonotic risk, emphasizing the need for reliable serological tools for epidemiological surveillance and vaccine evaluation. Here, we developed a double-antigen sandwich enzyme-linked immunosorbent assay (DAgS-ELISA) based on the PDCoV S1 protein, expressed in CHO cells and used as both coating and HRP-conjugated antigen. The assay reliably detected PDCoV-specific antibodies in sera from pigs, chickens, rabbits, and mice, showing high sensitivity (92.86%) and specificity (99.11%) as determined by receiver operating characteristic curve analysi

SUBMITTER: Bai Y 

PROVIDER: S-EPMC12718270 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature