The subcellular architecture of <i>Paratrypanosoma confusum</i> revealed by CryoET: A window into early trypanosome evolution.

ABSTRACT: The trypanosomatid Paratrypanosoma confusum represents a key evolutionary link between free-living bodonids and parasitic trypanosomes, yet its cellular architecture remains poorly characterized. Here, we used cryofocused ion beam scanning electron microscopy to prepare lamellae for cryoelectron tomography, enabling nanometer-scale imaging of its ultrastructure in near-native conditions. Our analysis revealed structures never before described in trypanosomatids, including an elaborate cytoskeletal network surrounding the flagellar pocket (FP), desmosome-like connections linking the FP membrane to the flagellum, and a cytopharyngeal-like invagination arising from the pocket. Luminal particles inside the FP were reconstructed in 3D, providing insight into their organization and potent

SUBMITTER: Alcantara CL 

PROVIDER: S-EPMC12718327 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature