Glycoengineering the Pseudomonas exotoxin A for multi-sequon integration and enhanced bioconjugation efficiency by PglS.

ABSTRACT: Glycoconjugate vaccines, also known as polysaccharide protein conjugate vaccines, consist of bacterial polysaccharides covalently linked to immunogenic carrier proteins. Bioconjugate vaccines are a type of glycoconjugate produced by oligosaccharyltransferases that catalyze the en bloc transfer of polysaccharides to specific amino acid motifs, called sequons, engineered into carrier proteins. Designing carrier proteins that are highly glycosylated by a specific oligosaccharyltransferase is critical for scalable bioconjugation platforms. Here, we describe the development of improved Pseudomonas aeruginosa exotoxin A (EPA) carrier proteins for glycosylation by the Acinetobacter baylyi ADP1 O-linking oligosaccharyltransferase PglS. Using a structure-guided approach, we integrated sequons at th

SUBMITTER: Knoot CJ 

PROVIDER: S-EPMC12718407 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature