Nutrient-driven regulation of saxitoxin gene expression and toxin production in Raphidiopsis raciborskii (Cyanobacteria).

ABSTRACT: Raphidiopsis raciborskii is a diazotrophic cyanobacterium, globally distributed in aquatic environments and known for forming toxic blooms, thereby affecting ecosystem services. South American strains are producers of saxitoxins, potent neurotoxins harmful to humans and animals. This study examined the effect of nutrient availability on saxitoxin production in two toxic R. raciborskii strains. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to investigate the transcriptional response of the saxitoxin sxtA4 gene under nitrogen and phosphorus gradients and the intracellular toxin concentration was measured by high performance liquid chromatography (HPLC). Results showed that the sxtA4 gene expression was generally upregulated at lower nutrient conditions. Posi

SUBMITTER: Zare M 

PROVIDER: S-EPMC12718436 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature