Polarity reversal of stable microtubules during neuronal development.

ABSTRACT: Long-distance transport in neurons relies on motor proteins that can move towards either the plus- or minus-end of microtubules. In axons, microtubules uniformly have a plus-end-out orientation, whereas dendrites of vertebrate neurons contain mixed polarity bundles: stable microtubules are typically minus-end-out, and dynamic microtubules are plus-end-out. This organization supports selective transport, yet how this dedicated microtubule organization is established is unclear. Here, we use single-molecule localization microscopy, expansion microscopy and live-cell imaging to examine how the microtubule cytoskeleton is reorganized during neuronal development in cultured rat hippocampal neurons. We find that early neurites contain mixed polarity microtubules, with stable microtubules initial

SUBMITTER: Iwanski MK 

PROVIDER: S-EPMC12718633 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature