Digital polymerase chain reaction quantification of hematopoietic chimerism by insertion/deletion: A personalized selection for different chimerism status.

ABSTRACT:

Background

Clinical decision-making after allogeneic stem cell transplantation (HSCT) is partially based on hematopoietic chimerism analysis. Short tandem repeat (STR), the current gold standard for quantitative chimerism analysis, has limited sensitivity. Digital polymerase chain reaction (dPCR) offers precise quantification and high reproducibility with excellent sensitivity (usually ≤0.1%) across a wide measurement range. However, the reported dPCR-based chimerism detection methods were developed in non-Chinese cohorts and may not be directly applicable to the Chinese population.

Methods

To improve sensitivity and accuracy, we first screened out 14 insertions/deletions (indel) loci with high individual recognition rates in Asian populations based on literature and NCBI da

SUBMITTER: Li DY 

PROVIDER: S-EPMC12718902 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature