Bacteria-inoculated biochar boosts copper immobilization and carbon sequestration via microbial restructuring and pH regulation.

ABSTRACT: Intensive agriculture aggravates soil acidification and heavy metal contamination. Biochar and its composites are cost-effective, carbon-rich amendments that can influence both heavy metal and soil carbon cycling, yet their synergistic mechanisms remain unclear. In this study, a combination of the diffusive gradient in thin films (DGT) technique, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS), and high-throughput sequencing was applied to investigate the effects of various biochar-based amendments: plain biochar, bacteria-inoculated biochar, and biochar combined with oyster shell powder on copper (Cu) stabilization and carbon dynamics in acidic orchard soils. All amendments significantly raised soil pH (by 1.03-2.54 ​units) and organic matter content, while reducin

SUBMITTER: Rao L 

PROVIDER: S-EPMC12719158 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature