SCPEP1⁺ basal cells are associated with the remodeling of oxidative stress signaling networks in idiopathic pulmonary fibrosis.

ABSTRACT:

Background

Idiopathic pulmonary fibrosis (IPF) is a chronic and fatal interstitial lung disease marked by progressive extracellular matrix accumulation and irreversible lung architecture remodeling. Oxidative stress (OS) plays a crucial role in IPF pathogenesis, yet its role across distinct cellular compartments and tissue microenvironments remains incompletely characterized.

Methods

We integrated single-cell RNA sequencing (scRNA-seq), spatial transcriptomics (stRNA-seq), and bulk RNA-seq datasets to comprehensively characterize oxidative stress activity across cellular and tissue scales in IPF lungs. Oxidative stress scores were calculated using multiple enrichment algorithms, and machine learning models (LASSO, Random Forest, Boruta, Bayesian, LVQ, Treebag) were applied t

SUBMITTER: Zhou X 

PROVIDER: S-EPMC12719287 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature