Multi-omics spatial characteristics of CD8⁺TRM cells in hepatocellular carcinoma and immunotherapy response prediction.

ABSTRACT:

Introduction

Understanding CD8⁺ tissue-resident memory T cells (TRM) spatial characteristics in hepatocellular carcinoma (HCC) is challenging, and clarifying the spatial feature changes following immunotherapy represents an urgent research gap.

Methods

This study employs a multi-omics approach to analyze the spatial distribution and intercellular interactions of TRM cells in HCC tissues using radiomics, single-cell sequencing, and multiplex immunofluorescence histochemistry (m-IHC).

Results

Our results show that the number of CD8⁺ TRM cells in HCC increases following immunotherapy. Furthermore, after dividing tumor tissues into the tumor core (TC), invasion margin (IM), and normal tissue (N), a increase in CD8⁺ TRM cells from the IM to

SUBMITTER: Li S 

PROVIDER: S-EPMC12719432 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature