Integrated genomics, metagenomics and metatranscriptomics to reveal the biocontrol mechanism of <i>Bacillus velezensis</i> JY10 against tobacco target spot disease.

ABSTRACT: Tobacco target spot (TTS) disease, a prevalent fungal disease caused by Rhizoctonia solani, severely reduces tobacco yield and quality, imposing substantial economic losses on the tobacco industry. In this study, we employed a biological control approach against TTS using a Bacillus velezensis JY10 isolated from healthy tobacco stems. We further elucidated the mechanism of JY10 in controlling TTS through genomics, metagenomics and metatranscriptomics. The results showed that JY10 exhibited robust inhibitory effects against R. solani, with an inhibition rate exceeding 95%, and achieved a TTS control efficacy of 68.63% in pot experiments. Whole-genome sequencing demonstrated that the JY10 genome spans 3,929,772 bp, contains 4,026 protein-coding genes, and has a GC conten

SUBMITTER: Yang W 

PROVIDER: S-EPMC12719468 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature