N-terminal half of MED14 is critical for Mediator-RNA polymerase II interaction and the resulting transcription.

ABSTRACT: Mediator is a large and evolutionarily conserved coactivator complex essential for RNA polymerase II (Pol II)-mediated gene regulation at multiple steps of the transcription process, including preinitiation complex assembly and function. Here, we used the MultiBac baculovirus expression system to generate recombinant human core Mediator subcomplexes and subsequent biochemical approaches to dissect the mechanism by which Mediator facilitates recruitment of Pol II to core promoters. Our results highlight a pivotal role in this process for the N-terminal half of the MED14 (MED14-NTD) subunit. We show that a reconstituted 15-subunit human core Mediator complex that contains only the MED14- NTD is fully functional in facilitating both basal and activated (p53) transcription. This complex direct

SUBMITTER: Baris Y 

PROVIDER: S-EPMC12719649 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature