A comparative study of NETO1 and NETO2 on channel-opening kinetics of GluK2 kainate receptors.

ABSTRACT: Neuropilin and tolloid-like proteins 1 and 2 (NETO1 and NETO2) are auxiliary subunits of kainate receptors, and GluK2 is a principal, pore-forming kainate receptor subunit. Kainate receptors are a subtype of ionotropic glutamate receptors. When coassembled with kainate receptors, NETO proteins are known to enhance the macroscopic current amplitude and affect channel properties such as channel desensitization rate. However, whether NETO proteins affect the rate of kainate receptor channel opening, which occurs in the microsecond time domain, is not known. Using a laser-pulse photolysis technique combined with whole-cell recording, we investigated the kinetic mechanism of channel opening of GluK2 homomeric receptors coexpressed with NETO1 and separately with NETO2 in human embryonic kidney-2

SUBMITTER: Saunders N 

PROVIDER: S-EPMC12719658 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature