Establishment of a stable replicon cell line of Tembusu virus (TMUV) for high-throughput antiviral screening.

ABSTRACT: Tembusu virus (TMUV), a member in the family Flaviviridae, induces egg-drop syndrome and fatal encephalitis in ducks, and thus threatens severely poultry industry. The development of preventive strategies against TMUV infection may contribute significantly to the viral disease control, but specific therapeutics is not currently available for TMUV infection. In this study, a novel TMUV replicon system was constructed in two cell lines by replacing viral structural genes with a Gaussia luciferase (Gluc) reporter and a blasticidin (BSD) resistance marker. Through continuous BSD selection, a stable Vero cell line harboring self-replicating TMUV RNA was established with replication fidelity over at least ten serial passages. Further whole-genome sequencing revealed that two adaptive mutations (

SUBMITTER: Song JW 

PROVIDER: S-EPMC12719727 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature