RING-Type E3 Ligase OsRFPH2-6 Ubiquitinates Nuclear Factor Y Subunit OsNF-YA1 to Suppress Rice Immunity.

ABSTRACT: RING-type E3 ubiquitin ligases play crucial roles in plant immunity by ubiquitinating their downstream substrates for degradation. We previously revealed, using CRISR/Cas9-mediated knockout mutant lines, that the RING-H2 E3 ubiquitin ligase OsRFPH2-6, transcriptionally regulated by the NAC transcription factor ONAC083, negatively modulates rice blast resistance. In this study, we further confirmed the negative role of OsRFPH2-6 in rice immunity through overexpression lines and investigated its underlying regulatory mechanism. OsRFPH2-6 possesses E3 ubiquitin ligase activity, which depends on each cysteine residue within its RING domain. OsRFPH2-6 interacts with and ubiquitinates OsNF-YA1, a subunit of nuclear factor Y transcription factor, leading to its degradation. Knockout of OsNF-YA1 a

SUBMITTER: Bi Y 

PROVIDER: S-EPMC12719848 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature