Poly-ADP-ribosylation modulated by poly(ADP-ribose) polymerase 1 is associated with glucose metabolism in colorectal cancer cells.

ABSTRACT: The preference of cancer cells to generate energy from glycolysis for rapid cell proliferation is called the Warburg effect. Poly(ADP-ribose) polymerase 1 (PARP1) performs various cellular functions, including poly-ADP-ribosylation and DNA repair. In the present study, we investigated the novel effects and mechanisms of PARP1 inhibition on glucose metabolism in colorectal cancer cells under hypoxia. We subjected Caco-2 and LoVo cancer cell lines to a concentration gradient of PARP1 inhibitor in a hypoxic environment induced with a tri-gas incubator (5 % CO₂, 1 % O₂, 94 % N₂). Inhibiting PARP1 activation attenuated Poly-ADP-ribosylation, increasing the NAD⁺/NADH ratio. High concentrations of PARP1 significantly reduced the glucose consumption rate

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12720136 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature