Autophagy-Induced Microglial Death Contributes to Neuroinflammation in Acute Optic Nerve Injury.

ABSTRACT:

Purpose

Optic neuropathy is a leading cause of blindness. Autophagy activation has been reported following optic nerve injury, but its precise role remains unclear. We investigated the involvement of autophagy in the early stages of optic nerve injury and explored potential therapeutic strategies.

Methods

A mouse optic nerve crush (ONC) model was established. Autophagy levels were assessed at different time points using electron microscopy and markers LC3 and Beclin-1. The relationship between autophagy and cell death was examined by Beclin-1 co-localization combined with TUNEL. Receptor-interacting protein (RIP3) knockout mice were used to determine its regulatory role in microglial autophagy-related cell death. Finally, the neuroprotective effect of inhibiting this pathway

SUBMITTER: Ke S 

PROVIDER: S-EPMC12720187 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature