LPS induces limited activation of hypoxia-inducible factor-1α in macrophages.

ABSTRACT: Hypoxia-inducible factor-1α (HIF-1α) plays a crucial role in cellular and tissue adaptation to low oxygen conditions. Although inflammatory stimuli such as lipopolysaccharide (LPS) also increase HIF-1α levels under normoxia, its transcriptional activity and regulatory mechanisms in this context remain unclear. To address this, we performed chromatin immunoprecipitation sequencing and transcriptome analyses in murine macrophages stimulated with either LPS or hypoxia. Both stimuli stabilized HIF-1α protein but via distinct mechanisms: hypoxia acted post-translationally, whereas LPS increased Hif-1α mRNA expression. Genome-wide HIF-1α binding was observed under both conditions; however, only hypoxia induced broad transcriptional activation of target genes, whereas LPS upregulated a restricted

SUBMITTER: Isagawa T 

PROVIDER: S-EPMC12720325 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature