RePol: A high-throughput screen for optimizing membrane protein solubilization and purification using polymers.

ABSTRACT: Extraction and purification of membrane proteins has for a long time represented a significant challenge. Polymer-based extraction methods, like those using styrene maleic acid co-polymers have provided a fertile approach to generate samples that include the local lipid environment surrounding the protein. However, the wide variety of different polymers now available provides a challenge to identify the optimal solution. In this study we develop and demonstrate a novel high-throughput screening approach for rapid optimization of polymer solubilization agents and chromatography resins for membrane protein purification. Using this approach, we explore whether there are standard conditions that perform well for a range of membrane protein morphologies, sources and functions. These data show t

SUBMITTER: Evans A 

PROVIDER: S-EPMC12720780 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature