Selection and evaluation of reference genes for quantitative real-time polymerase chain reaction normalization in Pieris melete (Lepidoptera, Pieridae).

ABSTRACT: Accurate normalization of gene expression data in real-time quantitative polymerase chain reaction (RT-qPCR) relies on the identification of stably expressed reference genes, which remains uncharacterized in Pieris melete Ménétriés, a cruciferous crop pest with agricultural significance. This study systematically evaluated eight candidate reference genes (GAPDH, α-tub, β-actin, 18S, β-tub, EF1α, RPL27, RPS15) across four experimental conditions: developmental stages, tissues, temperature stresses, and diapause stages. Stability rankings were determined using four algorithms (geNorm, NormFinder, BestKeeper, ΔCt method) integrated via RefFinder. Results revealed that at different condition-specific stability patterns, RPL27 and EF1α were optimal for developmental stages, RPL27 paired with 18

SUBMITTER: Jiang T 

PROVIDER: S-EPMC12721080 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature