TRBC1/TRBC2 Immunophenotyping Provides Added Value During the Diagnostic Workup of T-Cell Lymphoma.

ABSTRACT:

Background

Molecular T-cell receptor (TCR) clonality testing cannot link clonality to immunophenotype, limiting discrimination between malignant and clonal reactive T-cell expansions.

Methods

We evaluated TRBC1/TRBC2 immunophenotyping in 50 consecutive cases with suspected T-cell malignancy, assessing restriction (ratio > 10) across T-cell subsets.

Results

TRBC immunophenotyping showed 100% sensitivity for T-cell malignancy if assessed within subsets or phenotypically abnormal populations. Malignant clones predominantly showed CD4αβ/CD8αβ restriction (18/21), while reactive clones predominantly showed γδ/dual-positive/dual-negative restriction (15/16), enabling enhanced discrimination.

Conclusion

TRBC immunophenotyping provides sensitive clonality detection wit

SUBMITTER: Taylor J 

PROVIDER: S-EPMC12721119 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature