THBS1 identificated as an endometriosis biomarker through evidence from single-cell and bulk transcriptomic profiling.

ABSTRACT: Endometriosis affects a substantial number of women of reproductive age, yet current diagnostic methods rely on invasive procedures. To address this limitation, we investigated THBS1 as a potential biomarker and regulator of disease progression. Integrating bulk and single-cell RNA sequencing data, we identified THBS1 as a gene enriched in ectopic endometrial tissues. Immunohistochemistry confirmed its elevated expression, while functional assays revealed that THBS1 promotes endometrial cell proliferation, migration, and invasion. In a subcutaneous xenograft model, THBS1 silencing by small interfering RNA suppressed ectopic lesion growth, demonstrating its functional relevance in vivo. These findings position THBS1 as a central modulator of endometrial cellular behavior and provide

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12721194 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature