Single-Cell Multi-Omics Reveals B2M-Mediated Myeloid Reprogramming and Constructs a Predictive Model for Early Hepatocellular Carcinoma Recurrence.

ABSTRACT: Early recurrence remains a major challenge in the management of hepatocellular carcinoma (HCC), yet its molecular mechanisms are not fully understood. In this study, we applied an integrative multi-omics strategy at single-cell resolution to explore potential drivers of early HCC recurrence (recurrence time < 2 years) and to develop a predictive framework. By combining single-cell RNA sequencing, proteomics, transcriptomics, and clinical feature analysis, we identified 14 relapse-associated proteins, including CD274, B2M, MYC, and CASP3, as candidate risk factors. Transcriptomic profiling suggested the enrichment of pathways such as MYC-TARGETS-V2 and INTERFERON-GAMMA-RESPONSE. Single-cell analysis indicated reduced immune cell infiltration in recurrent tumors, with myeloid cells (particul

SUBMITTER: Tan Z 

PROVIDER: S-EPMC12721209 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature