Automated Label-Free Assay for Viral Detection and Inhibitor Screening via Biomembrane-Functionalized Microelectrode Arrays.

ABSTRACT: Most virus infection assays have indirect readout such as virus number following entry (e.g., PCR, cell lysis). While effective, these technologies are labor-intensive, require specialized environments (e.g., sterile or RNA-free), and detect later-stage viral events like lysis or cell death, lacking sensitivity to early fusion events. To address these limitations, we present biologically relevant 2D membrane materials, host-cell-derived supported lipid bilayers (hcd-SLBs), integrated with organic microelectrode arrays (OMEAs) for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) fusion. By overexpressing angiotensin-converting enzyme 2 (ACE2) receptors on the native membranes, the platform functions as a viral sensor capable of detecting virus pseudo particles (VPPs

SUBMITTER: Lu Z 

PROVIDER: S-EPMC12721210 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature