Intramacrophage RIL-seq uncovers an RNA antagonist of the Salmonella virulence-associated small RNA PinT.

ABSTRACT: Salmonella virulence chiefly relies upon two major pathogenicity islands, SPI-1 and SPI-2, which enable host cell invasion and intracellular survival, respectively. Growing evidence suggests post-transcriptional control of SPI gene expression by Hfq-dependent small regulatory RNAs (sRNAs) such as PinT. This 80-nucleotide sRNA is highly expressed after Salmonella enters host cells and modulates the transition from the SPI-1 to SPI-2 program by targeting the mRNAs of different virulence factors. However, it remains unclear how PinT activity can be counteracted when the suppression of virulence genes needs to be relieved. Here, we mapped the RNA interactome of Salmonella recovered from infected macrophages, using an optimized version of RIL-seq. In addition to offering an unprecedented view o

SUBMITTER: Kooshapour H 

PROVIDER: S-EPMC12721325 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature