Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetric <i>in vivo</i> imaging.

ABSTRACT:

Significance

Rapid acquisition of high-resolution volumetric images has been critical to effectively monitor dynamic biological processes in vivo, yet it faces tradeoffs between image resolution, penetration depth, and imaging speed. These limitations hinder the ability to study rapid neurophysiological events such as cerebrovascular dynamics and cellular activity, highlighting the need for advanced high-speed 3D imaging system.

Aim

To address these challenges in volumetric imaging performances, we aimed to develop a high-speed volumetric imaging system capable of resolving fast biological dynamics with minimal compromise in spatial resolution or imaging depth.

Approach

We devised a rapid axially scanned and de-scanned (RASAD) scheme by integrating a TAG lens (

SUBMITTER: Duy Mac K 

PROVIDER: S-EPMC12721343 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature