Expanding the Yeast MoClo Toolkit: gene expression control parts for <i>Saccharomyces cerevisiae</i> tested in industrially relevant conditions.

ABSTRACT: Fine-tuning of gene expression is often required to achieve competitive production levels in microbial cell factories. Several orthogonal expression systems based on heterologous regulatory parts have been developed for Saccharomyces cerevisiae. In laboratory conditions the systems demonstrate predictable results, but few expression systems have been tested in industrial conditions. Here, a new expression system based on the bacterial gene cusR was developed for S. cerevisiae, and two previous developed systems, the strong Bm3R1-based system and the quinic acid inducible Q-system, were adapted for compatibility with the Yeast MoClo Toolkit. The bacterial transcription factors CusR and Bm3R1 acted as DNA binding domains, and fused to a viral activation domain, they func

SUBMITTER: Blick E 

PROVIDER: S-EPMC12721388 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature