Apoptosis, Cell Cycle and Differentiation Effects of Propolis Extracted in Olive Oil on HL-60 AML Cells: A Flow Cytometric Analysis.

ABSTRACT: In recent years, researchers have focused on the drug capacity of natural products and their components with low side effects in the treatment of leukemia. This study aims to elucidate the antileukemic mechanism of action of olive oil extract of propolis (OEP) together with methylprednisolone (MP) in HL-60 cells, an acute myeloid leukemia cell line. The WST-8 kit was used to test the cytotoxic and proliferative effects of OEP on HL-60 cells. Flow cytometry was additionally applied for determining apoptotic status, cell cycle, and differentiation levels (CD11b, CD14, and CD68). The IC₅₀ concentration of OEP in HL-60 cells was determined to be 0.5 μ L/mL after a 24 h period. OEP effectively arrested the cell cycle at the G₂/M phase after 24 h, and its addition of

SUBMITTER: Dogan H 

PROVIDER: S-EPMC12721472 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature