Structural basis for collagen recognition by the <i>Streptococcus pyogenes</i> M3 protein and its involvement in biofilm.

ABSTRACT: The M protein is an essential virulence factor of Streptococcus pyogenes, or group A streptococcus (GAS), one of the most common and dangerous human pathogens. Molecular and functional characterization of M protein variants and their interactions with host components is crucial for understanding streptococcal pathogenesis and vaccine development. The M3 protein is produced by the prevalent emm3 GAS serotype, which is frequently associated with severe invasive diseases. Here, we structurally and biochemically characterize the interaction of M3 with human collagens. High-resolution structures of the N-terminal M3 domain in the free state as well as bound to a collagen peptide derived from the Collagen Ligands Collection reveal a novel T-shaped protein fold that presents binding

SUBMITTER: Wojnowska M 

PROVIDER: S-EPMC12721709 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature