Irisin activates the AMPK-Beclin1 signaling pathway to regulate pulmonary autophagy induced by CSE + LPS.

ABSTRACT: Smoking and infection are the most common risk factors for acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Irisin is a hormone-like substance that helps reduce oxidative stress and inflammation in lung diseases. This study aims to explore the potential regulatory mechanism of irisin in AECOPD induced by CSE combined with LPS. Autophagy-deficient models were established in mice and MH-S cells. Pathological changes in mouse lung tissues were assessed using HE staining, while Irisin expression was detected via immunofluorescence. Levels of IL-1β, IL-6, and TNF-α in mouse BALF and MH-S cell supernatants were measured by ELISA. Autophagic flux in MH-S cells was monitored using the Ad-mCherry-GFP-LC3B dual-fluorescence system and transmission electron microscopy. Western bl

SUBMITTER: Chen Z 

PROVIDER: S-EPMC12722212 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature