Scarless circular mRNA-based CAR-T cell therapy elicits superior antitumor efficacy.

ABSTRACT: Messenger RNA (mRNA)-based transient expression of chimeric antigen receptors (CARs) results in optimal safety profiles and provides promising opportunities to address existing challenges associated with viral vector-based CAR-T-cell therapies and to meet emerging medical needs for noncancerous indications. Conventional linear mRNAs, however, are intrinsically unstable and typically support short-lived protein expression, which can constrain therapeutic activity. Here, we engineered a high-efficiency permuted intron exon (PIE) platform to synthesize scarless circular mRNAs (cmRNAs) that drive robust CAR expression with extended durability. The scarless design avoids extraneous junction sequences, streamlining manufacturability and potentially reducing innate immune sensing. Compared with l

SUBMITTER: Hu Q 

PROVIDER: S-EPMC12722250 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature