Expression and regulation network of BLNK in CP/CPPS in animal, cell model and clinical samples.

ABSTRACT: B-cell linker (BLNK) is associated with the inflammatory process of certain diseases. However, no studies have explored the role of BLNK in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). We performed transcriptome analysis of the rat model and found that BCR signaling pathway, especially the BLNK gene, is closely related to CP/CPPS. We detected the expression levels of BLNK gene or protein in CP/CPPS rat tissues, human expressed prostatic secretion (EPS), and cell models by qPCR, WB, and IHC. Subsequently, we silenced the BLNK gene in human prostatitis cell model. The cell cycle, and expression level of inflammatory factors were detected; the core pathway and interacting molecules of BLNK were revealed by mRNA/lncRNA sequencing, bioinformatics analysis and validation. Results

SUBMITTER: Yu P 

PROVIDER: S-EPMC12722277 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature