Kinetochore mutations and histone phosphorylation pattern changes accompany holo- and macro-monocentromere evolution.

ABSTRACT: Centromeres are essential for kinetochore assembly and spindle attachment. While chromosomes of most species are monocentric with a single centromere, a minority exhibit holocentricity, with a centromere along the chromatid length. Sporadic emergence of holocentricity suggests multiple independent transitions. To explore this, we compare the centromere and (epi)genome organization of two sister genera with contrasting centromere types: Chamaelirium luteum with large macro-monocentromeres and Chionographis japonica with holocentromeres. Both exhibit chromosome-wide histone phosphorylation patterns distinct from typical monocentric species. Kinetochore analysis reveals similar chimeric Borealin in both species, with additional KNL2 loss and NSL1 chimerism in Cha. luteum. The broad-scale synt

SUBMITTER: Kuo YT 

PROVIDER: S-EPMC12722358 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature