AR Deficiency Protects Against Sepsis-Induced Acute Lung Injury by Inhibiting Macrophage M1 Polarization and Inflammatory Cytokine Secretion.

ABSTRACT: Acute lung injury (ALI) constitutes a major public health concern, with sepsis contributing to up to 50% of ALI cases in the ICU. Androgen receptor (AR) plays a crucial role in immunoregulation. Our study aimed to explore the role of AR and the underlying regulatory mechanisms of AR signaling in septic ALI. We assessed the role of AR in a septic ALI model by manipulating AR expression through orchiectomy, AR knockout, and enzalutamide treatment. Lung injury was evaluated by HE staining and measuring pulmonary vascular leakage. Inflammatory cell infiltration and inflammatory mediator levels were assessed through Immunofluorescence staining and western blot. Additionally, Weighted Gene Co-expression Network Analysis (WGCNA), lentiviral vector, chromatin immunoprecipitation and co-immunopreci

SUBMITTER: Wang X 

PROVIDER: S-EPMC12722362 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature