Zmiz1-Mediated SUMOylation of NLRP3 Inflammasome Regulates Satellite Glial Cell Activation and Neuronal Autophagy in Trigeminal Neuralgia.

ABSTRACT: Trigeminal neuralgia (TN) is characterized by neuroinflammation and satellite glial cell (SGC) activation, but the molecular mechanisms remain unclear. This study identifies zinc finger MIZ-type containing 1 (Zmiz1) as a key regulator that promotes SUMOylation of NLRP3 inflammasome, thereby influencing SGC activation and neuronal autophagy in TN. Using bioinformatics analysis, we identified Zmiz1 as a key SUMOylation-related gene involved in TN. Single-cell transcriptomics and co-expression network analysis revealed Zmiz1 enrichment in SGCs and neurons. Co-immunoprecipitation (Co-IP) and western blotting confirmed Zmiz1's interaction with NLRP3 and its role in promoting NLRP3 SUMOylation. In vitro experiments assessed the impact of Zmiz1 overexpression and knockdown on SGC activation, infl

SUBMITTER: Jing F 

PROVIDER: S-EPMC12722413 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature