Maternal provisioning and zygotic activation: transcriptomic dynamics of early atlantic halibut (<i>Hippoglossus hippoglossus</i>) embryogenesis.

ABSTRACT:

Introduction

In Atlantic halibut (Hippoglossus hippoglossus) aquaculture, egg and larval quality remain major bottlenecks. Most transcriptomic and proteomic studies compare freshly fertilized eggs of good versus poor quality, assuming early molecular differences explain hatching success. However, many lethal phenotypes arise only after the mid-blastula transition (MBT), suggesting that early comparisons may overlook key developmental processes.

Methods

We performed stage-resolved RNA sequencing of Atlantic halibut embryos spanning unfertilized egg (UF), cleavage (8C, 16C), blastula (BL), 25% epiboly (25 EB), and blastopore closure (50 degree-days, 50 dd). Differential expression analysis (DESeq2 with LFC shrinkage), Gene Ontology (GO) overrepresentation (PANTHER, REVI

SUBMITTER: Niepagen N 

PROVIDER: S-EPMC12722812 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature