The microbiome exists in the neuroretina and choroid in normal conditions and responds rapidly to retinal injury.

ABSTRACT:

Purpose

To investigate the microbial profiles in the retina and RPE/choroid, and how they respond to retinal injury.

Methods

Adult C57BL/6J mice were subjected to retinal laser burns using a photocoagulator. One and 24h later, the retina and RPE/choroid were collected under strict sterile conditions and processed for 16S rRNA paired-end sequencing (2×250). The data were analyzed using R software, GraphPad Prism, OmicShare, and Wekemo Bioincloud.

Results

Microbiota were detected in the retina and RPE/choroid under normal physiological conditions. The alpha diversity was higher in the retina than in the RPE/choroid. All retinal microbiotas at the phylum level and 12 out of 14 at the genus level were shared with those of RPE/choroid. The top phyla were Firmicutes,

SUBMITTER: Cui X 

PROVIDER: S-EPMC12722843 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature