Antihypertensive and antioxidant peptide identification and storage stability assessment of novel probiotic fermented sheep milk: A comparative study with bovine milk.

ABSTRACT: This study investigated angiotensin-converting enzyme (ACE) inhibitory activity (ACE-IA), antioxidant activity (AnOxA), and bioactive peptides in novel probiotic fermented sheep and bovine milks, along with their storage stability and sensory properties over 28 days at 4 °C. Milks fermented by Lactobacillus delbrueckii MF55 exhibited strong ACE-IA and AnOxA, whereas Lacticaseibacillus paracasei MF48 produced only pronounced AnOxA. After ultrafiltration, QExactive identification, in silico analysis, and molecular docking simulations, four novel peptides with antihypertensive and antioxidant potential (ITVDDKHYQK, AMKPWTQPK, YPVEPFTER, KYNVPQLEIVPK) were screened from the <3 kDa fractions. Among them, AMKPWTQPK and KYNVPQLEIVPK were uniquely detected in fermented sheep m

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12722972 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature