TSC1 deficiency drives immune evasion in colorectal cancer via mTORC1-mediated dysregulation of PD-L1 sialylation.

ABSTRACT:

Background

TSC1 serves as a critical regulator of the mTORC1 signaling pathway with established roles in colorectal cancer pathogenesis. This investigation systematically examined the clinical relevance of TSC1 in colorectal cancer and its mechanistic relationship with sialylation-mediated immune regulation through integrated analysis of TCGA datasets and experimental validation.

Methods

We employed bioinformatic analysis of TCGA cohorts combined with in vitro and in vivo experimental models. Molecular mechanisms were interrogated using biochemical assays, transcriptional profiling, and targeted pathway interventions. Sialylation dynamics were quantified through lectin-binding assays and surface plasmon resonance analysis.

Results

Clinical analysis revea

SUBMITTER: Guan X 

PROVIDER: S-EPMC12723005 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature