Single-cell transcriptomics reveals immune remodeling of the murine lung microenvironment following chronic house dust mite exposure.

ABSTRACT:

Introduction

House dust mite (HDM) is a common environmental aeroallergen strongly associated with asthma and chronic airway inflammation. While HDM exposure is known to induce T helper 2 (Th2)-mediated eosinophilic inflammation, its chronic effects on interleukin-1β (IL-1β)-associated neutrophilic inflammation remain poorly understood. This study used single-cell RNA sequencing (scRNA-seq) to investigate how chronic HDM exposure remodels the murine lung immune microenvironment, with a focus on the role of IL-1β in shaping HDM-driven immune responses.

Methods

Wild-type and Il1b-deficient mice on a C57BL/6 background were subjected to chronic HDM exposure over a 5-week period, and scRNA-seq was performed to characterize changes in the lung immune landscape. To enhance

SUBMITTER: Chang H 

PROVIDER: S-EPMC12723145 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature