Knockdown of LncRNA CCAT1 Attenuates ox-LDL-Induced Inflammation in THP1-Derived Macrophages via the miR-296-3p/FOSL1 Axis.

ABSTRACT: Macrophage-driven inflammation, induced by dysfunctional lipid metabolism, is a pivotal process in the pathogenesis of atherosclerosis (AS). While long noncoding RNAs (LncRNAs) are established regulators, the specific mechanisms governing their roles remain largely uncharacterized. Here, we demonstrate that the LncRNA colon cancer-associated transcript 1 (CCAT1) is significantly upregulated in THP1-derived macrophages upon stimulation with oxidized low-density lipoprotein (ox-LDL). Silencing LncRNA CCAT1 markedly attenuated the ox-LDL-induced inflammatory response, establishing its proinflammatory function. Mechanistically, we identified miR-296-3p as a direct downstream target of LncRNA CCAT1, which acts as a molecular sponge. This interaction was validated by dual-luciferase and RNA pull

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12723178 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature