Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis.

ABSTRACT: Cilia protrude from the cell surface to execute signal transduction and cell motility. Tectonic (TCTN) is a transition-zone (TZ) "gatekeeper" that determines ciliary composition and function. The role of its only conserved module in the TCTN protein family, DUF1619, lacks functional annotation. Herein, we quantitatively compared full-length TCTN1 and four truncations (DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C) stably expressed in Chlamydomonas reinhardtii tctn1 cells. Surprisingly, neither fragment could rescue ciliary morphology of tctn1; DUF1619 alone aggravates ciliary defects. In particular, deletion of either terminus decreases TZ localization and, for the C-terminus, destabilizes the protein during ciliary disassembly. Thus, our findings demonstrate that the full-leng

SUBMITTER: Yu H 

PROVIDER: S-EPMC12723291 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature