A multifunctional fungal defensin bladesin loaded-hydrogel for accelerated infectious wound healing.

ABSTRACT: Wound management is significantly challenged by drug-resistant bacterial infections. In this study, a novel fungal defensin peptide derived from Blastomyces dermatitis was identified. The full-length peptide named bladesin was composed of 116 amino acids containing three regions: signal peptide, pro-peptide, and mature peptide. Sequence and structural analysis revealed that the linear mature peptide (reduced type) was consisted of 38 amino acids. The mature bladesin contains three intramolecular disulfide bonds (Cys4-Cys29, Cys14-Cys35 and Cys18-Cys37) and can form a characteristic cysteine-stabilized alpha-beta motif in the native state. In vitro studies demonstrated it exhibited potent antibacterial activity against Gram-positive pathogens with minimum inhibitory concentrat

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12723373 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature